In the EDIM detect, the markers TKTL1 and Apo10 are used.
Both biomarkers detect fundamental biophysical mechanisms that are significantly altered in all forms of malignancy (Grimm et al., 2013).
All forms of tumour cells exhibit a disorder in the process of the planned cell death (apoptosis). Only when this process is disturbed and as a result of which the cell no longer kills itself can a tumour cell form. This apoptosis can be detected with Apo10.
TKTL1 shows the change of energy generation from oxidative phosphorylation to fermentative energy release. This is also a fundamental biophysical process that can be used by all tumour cells, regardless of their entity. The activation of the TKTL1 gene in tumour cells leads to a fermentative, oxygen-independent metabolism which is accompanied by an increased uptake of glucose, an enhanced formation of lactic acid and the capacity for invasive growth.
The meaning and function of TKTL1 has been clearly demonstrated with the latest publication (Jayachandran et al., 2016 / Diaz-Morali et al., 2016).
- Jayachandran, A.; Lo, P.H.; Chueh, A.C.; Prithviraj, P.; Molania, R.; Davalos-Salas, M.; Anaka, M.; Walkiewicz, M.; Cebon, J.; Behren, A.
Transketolase-like 1 ectopic expression is associated with DNA hypomethylation and induces theWarburg effect in melanoma cells.
BMC Cancer 2016, 16, 134. [PubMed]
- Diaz-Moralli, S.; Aguilar, E.; Marin, S.; Coy, J.F.; Dewerchin, M.; Antoniewicz, M.R.; Meca-Cortés, O.; Notebaert, L.; Ghesquière, B.; Eelen, G.; et al.
A key role for Transketolase-like 1 in tumor metabolic reprogramming.
Oncotarget 2016, 7, 51875–51897 [PubMed]
- Grimm, M.; Schmitt, S.; Teriete, P.; Biegner, T.; Stenzl, A.; Hennenlotter, J.; Muhs, H.J.; Munz, A.; Nadtotschi, T.; König, K.; et al.
A biomarker based detection and characterization of carcinomas exploiting two fundamental biophysical mechanisms in mammalian cells.
BMC Cancer 2013, 13, 569. [PubMed]